primary antibodies recognizing nf-κb, iκbα, p-iκbα (ser32), ikkβ, p-ikkβ, myd88, lamin b1, cox-2, inos, gapdh  (Cell Signaling Technology Inc)

Journal: Stem Cell Research & Therapy

Article Title: Knockdown of LAP2α inhibits osteogenic differentiation of human adipose-derived stem cells by activating NF-κB

doi: 10.1186/s13287-020-01774-9

Figure Lengend Snippet: Knockdown of LAP2α promotes translocation of p65 from the cytoplasm to nucleus. a Relative mRNA expression levels of p65 target genes IL-6 , ICAM1 , and TRAF1 , as measured by qRT-PCR, n = 6. b Proteins levels of p-IκBα, total IκBα, p-p65, and total p65 as measured by western blotting analysis in hASCs expressing shNC, shLAP2α-1, or shLAP2α-2. c Quantification of western blotting analysis, n = 3. d Immunofluorescent confocal microscopy of p65 nuclear translocation and LAP2α expression in hASCs expressing shNC, shLAP2α-1, or shLAP2α-2, treated or not treated with TNF-α for 30 min, scale bars: 50 μm. Data are shown as the mean ± SD; * P < 0.05 compared with the control group; ** P < 0.01 compared with the control group; NS: not significant

Article Snippet: The membranes were blocked and then incubated with primary antibodies recognizing GAPDH, RUNX2, p-p65 (Ser536), p-IκBα (ser32/ser36), and p65 (Cell Signaling Technology), and LAP2α (Abcam, Cambridge, MA, USA) at 4 °C overnight.

Techniques: Knockdown, Translocation Assay, Expressing, Quantitative RT-PCR, Western Blot, Confocal Microscopy, Control

Journal: Cell Death & Disease

Article Title: LINC01355 suppresses breast cancer growth through FOXO3-mediated transcriptional repression of CCND1

doi: 10.1038/s41419-019-1741-8

Figure Lengend Snippet: List of oligonucleotides used in the study

Article Snippet: The membranes were incubated with 5% fat-free milk at room temperature for 1 h to block nonspecific binding, and probed with the primary antibodies recognizing cyclin D1, FOXO3, and GAPDH (Cell Signaling Technology, Inc., Beverly, MA, USA).

Techniques: Sequencing, ChIP-qPCR, shRNA